# LINC01214 Promotes Non-Small Cell Lung Cancer Through the miR-497-3p/HSP90AB1 Axis

## TL;DR

This study shows that LINC01214 promotes non-small cell lung cancer by interacting with miR-497-3p and HSP90AB1, offering new insights for treatment.

## Contribution

The study identifies a novel regulatory axis involving LINC01214, miR-497-3p, and HSP90AB1 in NSCLC progression.

## Key findings

- LINC01214 levels are elevated in NSCLC tissues and correlate with poor prognosis.
- LINC01214 promotes cancer cell proliferation, migration, and invasion by targeting miR-497-3p and HSP90AB1.
- Silencing LINC01214 inhibits tumor growth and reverses the effects of miR-497-3p suppression.

## Abstract

This study aimed to explore the potential of LINC01214 in providing prognostic and therapeutic insights, thereby offering valuable references for the research of non-small cell lung cancer (NSCLC).

122 NSCLC subjects were recruited. The molecular level was quantified by qPCR and WB. Kaplan–Meier estimated the prognostic effects and Cox regression analyses the hazard ratio. The cell activities including proliferation, apoptosis, and migration/invasion were evaluated by CCK-8, flow cytometry, and Transwell. The regulatory axis of LINC01214/miR-497-3p/HSP90AB1 was verified by the dual luciferase reporter assay and rescue experiments.

LINC01214 increased both in the malignant tissues and cell lines of NSCLC. Mortality was increased in NSCLC patients with high LINC01214 levels. LINC01214 was an independent risk predictor of prognosis in NSCLC. Silencing of LINC01214 inhibited the NSCLC cell proliferation, migration, and invasion and promoted apoptosis. The abundance of miR-497-3p showed an opposite trend to LINC01214. LINC01214 could target miR-497-3p and negatively correlate with miR-497-3p. The inhibitory effect of LINC01214 on cell activity was reversed by miR-497-3p. HSP90AB1 was predicted and further confirmed as the target of miR-497-3p. The LINC01214/miR-497-3p/HSP90AB1 axis regulated NSCLC cell proliferation, migration, and invasion.

LINC01214, a potential biomarker, contributed to the progression of NSCLC through the miR-497-3p/HSP90AB1 axis by promoting cell proliferation and motility.