# Kinetics effect and inhibition mechanism of Tartary buckwheat extracts on acrylamide in the asparagine-glucose system

## TL;DR

Tartary buckwheat extracts, especially sprouts and rutin, significantly reduce acrylamide formation in food reactions.

## Contribution

The study identifies rutin as a key inhibitor and proposes three new pathways for acrylamide mitigation.

## Key findings

- Tartary buckwheat sprouts inhibit acrylamide by 47.95% at 1 mg/mL.
- Rutin achieves 64.69% inhibition at 10−9 mol/L.
- The Logistic-Exponential model best fits acrylamide kinetics.

## Abstract

This work investigated the kinetics effect and the inhibition mechanism of acrylamide (AA) formation by adding Tartary buckwheat extracts from Tartary buckwheat seeds (TB) and Tartary buckwheat sprouts (TBS) in the asparagine-glucose reaction system. TBS exhibited greater inhibitory activity against AA than TB, demonstrating inhibition rates of 47.95 % and 32.13 % at 1 mg/mL, respectively. Rutin, the main polyphenol of extracts, achieved the highest inhibition rate (64.69 %) at 10−9 mol/L. The Logistic-Exponential kinetic model was well fitted to the kinetic changes of AA in this study. In addition, the kinetics change of reaction products and oxidation-reduction potential indicated that Tartary buckwheat extracts and rutin might mitigate AA through three paths: 1) decrease the conversion of 3-aminopropionamide (3-APA) to AA, 2) reduce the formation of hydroxymethylfurfural (HMF), 3) enhance the elimination of AA by increasing the positive voltage of the model system.

•Tartary buckwheat extracts inhibit acrylamide formation in Maillard reaction.•TBS showed higher inhibition rates (47.95 %) than TB (32.13 %) at 1 mg/mL.•Rutin displayed the highest inhibition rate (64.69 %) at 10−9 mol/L.•Logistics-exponential model best fits acrylamide kinetic changes.•Inhibition pathways: 3-APA/HMF reduction and redox enhancement.

Tartary buckwheat extracts inhibit acrylamide formation in Maillard reaction.

TBS showed higher inhibition rates (47.95 %) than TB (32.13 %) at 1 mg/mL.

Rutin displayed the highest inhibition rate (64.69 %) at 10−9 mol/L.

Logistics-exponential model best fits acrylamide kinetic changes.

Inhibition pathways: 3-APA/HMF reduction and redox enhancement.