# TAOK2 controls synaptic plasticity and anxiety via ERK and calcium signaling

## TL;DR

TAOK2 is important for brain cell communication and behavior, and its loss causes problems in signaling and anxiety-like behavior in mice.

## Contribution

This study reveals that TAOK2 regulates synaptic plasticity and anxiety via ERK and calcium signaling in excitatory neurons.

## Key findings

- TAOK2 loss disrupts ERK/MAPK and calcium signaling in cortical neurons.
- TAOK2-deficient neurons show reduced synaptic density and connectivity.
- TAOK2 knockout mice display anxiety-related behavior in open field tests.

## Abstract

The kinase thousand and one amino acid kinase 2 (TAOK2) regulates dendritic architecture and synaptic plasticity and is implicated in neurodevelopmental and neuropsychiatric disorders, including autism and schizophrenia. Here, we investigated TAOK2 function by creating an Emx1-Cre-driven, excitatory-neuron-specific conditional Taok2 knockout (Taok2 cKO) mouse line. Pathway profiling in Taok2 cKO primary cortical neurons revealed impaired extracellular regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) and calcium signaling after AMPA, BDNF, or bicuculline stimulation. These results were validated by reduced p-ERK1/2 protein levels and decreased calcium flux. Cultured Taok2 cKO neurons displayed reduced synaptic density and connectivity. Single-nucleus RNA sequencing of medial prefrontal cortex identified dysregulated gene expression enriched for postsynaptic MAPK and calcium pathways within cortical layers 2/3 and 4/5. Taok2 cKO mice exhibited an anxiety-related thigmotactic behavior in the open field test. Our findings demonstrate that TAOK2 loss in excitatory cortical neurons disrupts synaptic signaling and connectivity, drives behavioral abnormalities, and positions TAOK2 as a potential drug target for neuropsychiatric disorders.

•TAOK2 loss in excitatory cortical neurons disrupts ERK/MAPK and calcium signaling•TAOK2-deficient neurons show reduced synaptic density and connectivity in culture•snRNA-seq reveals transcriptional changes in cortical postsynaptic signaling•Taok2 conditional knockout mice display anxiety-related behavior in open field test

TAOK2 loss in excitatory cortical neurons disrupts ERK/MAPK and calcium signaling

TAOK2-deficient neurons show reduced synaptic density and connectivity in culture

snRNA-seq reveals transcriptional changes in cortical postsynaptic signaling

Taok2 conditional knockout mice display anxiety-related behavior in open field test

Molecular neuroscience; Cellular neuroscience; Transcriptomics