# Specific auxin and medium combinations alter Saccharomyces cerevisiae growth

## TL;DR

This study shows that auxin and medium combinations affect yeast growth, suggesting the need for careful controls in experiments using auxin.

## Contribution

The novel finding is that auxin affects yeast growth independently of the degradation system, depending on the medium.

## Key findings

- Natural and synthetic auxin impair yeast growth in minimal but not rich media.
- Auxin effects are observed regardless of the auxin-inducible degradation system's presence.
- Proper controls are essential when using auxin-inducible degradation methods.

## Abstract

Auxin-inducible degradation is an increasingly popular protein-targeting reverse genetics approach. Use of this method has revolutionized the types of questions cell and molecular biologists can answer, however, a growing number of studies point to auxin alone impacting different cellular phenotypes. This study investigated the impact of different medium and auxin combinations on
Saccharomyces cerevisiae
growth. We observed that both natural and synthetic auxin (Indole-3-acetic acid (IAA) and 1-Naphthaleneacetic acid (NAA) respectively) impaired budding yeast growth in nutrient minimal but not nutrient rich media. This finding was true across different yeast strains with or without an intact auxin-inducible degradation system. Ultimately, this study highlights the need for proper controls when using auxin-inducible degradation.