# Reproducible 3D bioprinting of Streptococcus mutans to create model oral biofilms

## TL;DR

A new 3D bioprinting method was developed to create realistic oral biofilms using Streptococcus mutans, enabling better study of biofilm dynamics and virulence.

## Contribution

A novel 3D-bioprinting technique was developed to create model oral biofilms on biomimetic substrates, preserving virulence while enabling spatial control.

## Key findings

- Biofilms printed with bio-ink showed similar virulence factors to standard in vitro methods.
- The bio-ink method resulted in higher exopolysaccharide deposition and more uniform bacterial distribution.
- The new technique allows biofilm printing on any substrate, enhancing study of biofilm dynamics.

## Abstract

Novel approaches are needed to study relationships between oral biofilm strains, enable three-dimensional oral biofilm deposition, and hasten the rigor and pace of basic and translational biofilm studies. Previously, 3D-bioprinters were leveraged to deposit spatially patterned biofilms onto sugar-rich agar surfaces to study how the underlying spatial organization of various microbes impacts biofilm persistence and virulence. Herein, we have developed a new method to adapt this process from limited, soft agar surfaces to biomimetic solid substrates submerged in aqueous solutions for studying oral biofilms in vitro. Streptococcus mutans UA159 was used to compare standard in vitro biofilm development with our new 3D-printed bio-ink hydrogels on hydroxyapatite disks, which mimic tooth surfaces. Biofilms formed using the bio-ink methodology showed minimal quantitative differences in virulence factors, including environmental pH, biomass, and cell density, compared to biofilms formed using the standard in vitro methodology. The bio-ink technique resulted in higher exopolysaccharide deposition, a key virulence factor for biofilm cohesion and protection, as well as more homogeneous spatial distribution of bacterial microcolonies. Our newly developed technique produces 3D-printable model biofilms that match the virulence benchmarks of the standard method, opening possibilities to print biofilms onto any substrate and a new way to study multidimensional biofilm dynamics.

Dental caries is the most common oral disease caused by biofilms in humans with cost limitations. Changes in the human diet have increased the exposure to sugar-rich processed food, increasing the incidence and severity of dental caries and creating greater rationale for understanding biofilm deposition, microbial interactions, and maintenance of quiescence of the oral microbiota. Recent 3D-printing techniques have been leveraged to develop the first model biofilms, providing spatial control over microbe deposition and enabling unprecedented investigation of the impact of cell-cell interactions and spatial organizationupon biofilm persistence, sensitivity to drugs, and virulence. Here, we have developed new methods to extend bioprinting to oral biofilms using cariogenic Streptococcus mutans. Our technique is an attempt to establish an alternative method for oral biofilm formation in vitro that uses 3D-printing tools, preserving the virulence of standard in vitro biofilms while amplifying the availability and versatility of methods for understanding the microbiome.