# Scope and Synthetic Applications of the Aryl-Alcohol Oxidase from Streptomyces hiroshimensis (ShAAO)

## TL;DR

This paper explores a new enzyme from Streptomyces hiroshimensis that efficiently produces carbonyl compounds, useful in industry and research.

## Contribution

The study reveals the unique activity profile and industrial potential of ShAAO in synthesizing chiral compounds.

## Key findings

- ShAAO showed distinct activity across diverse alcohols compared to other AAOs.
- It enabled the synthesis of a paroxetine intermediate with high yields and turnover numbers over 65000.
- Immobilization improved ShAAO's stability for potential industrial use.

## Abstract

The sustainable production of carbonyl compounds is of
growing
interest due to their broad academic and industrial interest. Herein,
we explored the aryl-alcohol oxidase from Streptomyces hiroshimensis (ShAAO), mapping its activity across diverse alcohols,
revealing a distinct profile from other natural AAOs. Integrated into
tandem organocatalytic processes, ShAAO enabled the
synthesis of various chiral compounds, including an intermediate for
paroxetine. Gram-scale reactions delivered high yields and turnover
numbers exceeding 65000. Immobilization further enhanced stability,
highlighting ShAAO as an efficient and industrially
relevant biocatalyst.