Elastic analysis bridges structure and dynamics of an AAA+ molecular motor
Victor Hugo Mello, Jiri Wald, Thomas C Marlovits, Pablo Sartori, Arne Elofsson, Arne Elofsson, Arne Elofsson
TL;DR
This paper explains how a molecular motor called RuvB converts energy into motion by analyzing its structural changes and energy transformations.
Contribution
The study introduces a new method to compute residue-scale elastic pseudoenergy in proteins and applies it to understand the hand-over-hand mechanism in RuvB.
Findings
DNA binding in RuvB is associated with overcoming a high energy barrier.
Energy transmission between subunits drives the hand-over-hand mechanism in RuvB.
The method can integrate structural and biophysical data to model AAA+ motor dynamics.
Abstract
Proteins carry out cellular functions by changing their structure among a few conformations, each characterised by a different energy level. Therefore, structural changes, energy transformations, and protein function are intimately related. Despite its central importance, this relationship remains elusive. For example, while many hexameric ATPase motors are known to function using a hand-over-hand alternation of subunits, how energy transduction throughout the assembly’s structure drives the hand-over-hand mechanism is not known. In this work, we unravel the energetic basis of hand-over-hand in a model AAA+ motor, RuvB. To do so, we develop a general method to compute the residue-scale elastic pseudoenergy due to structure changes and apply it to RuvB structures, recently resolved through cryo-EM. This allows us to quantify how progression through RuvB’s mechanochemical cycle translates…
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Taxonomy
TopicsProtein Structure and Dynamics · Force Microscopy Techniques and Applications · Advanced Electron Microscopy Techniques and Applications