# Topology-controlled Pt atomic sites enhance electron utilization efficiency for NAD+ regeneration and alcohol detoxification

## TL;DR

This study introduces a new method for alcohol detoxification using Pt atomic sites in Co3O4 lattices to improve coenzyme regeneration and reduce harmful byproducts.

## Contribution

The novel use of topology-controlled Pt atomic sites enhances electron utilization and reduces ROS in alcohol metabolism.

## Key findings

- PtTdCo/O shows 3.59- to 3.83-fold higher NOX-like activity compared to other structures.
- PtTd sites improve substrate adsorption and lower reaction energy for alcohol degradation.
- The method minimizes ROS production while promoting efficient electron utilization.

## Abstract

Acute alcohol intoxication causes severe damage to the liver, nervous system and metabolic processes while inducing inflammatory responses. Current therapeutic strategies targeting ethanol metabolism through alcohol or aldehyde dehydrogenase activation face critical limitations of NADH accumulation and excessive reactive oxygen species (ROS) generation. Herein, by introducing a topology-controlled Pt atomic site into Co3O4 lattices, the geometric structure diversification of Pt was achieved for alcohol detoxification by coenzyme regeneration. In comparison with octahedral PtOhCo/O and pure Co3O4, PtTdCo/O (atomic Pt sites within tetrahedral topology) exhibits 3.59- and 3.83-fold higher NOX-like activity, and preferentially catalyzes 4e⁻ oxygen reduction, improving electron utilization while minimizing ROS production. Mechanistic studies indicate that the introduction of PtTd sites improves substrate adsorption, enhances electron utilization efficiency and lowers the energy of reactions, thereby promoting alcohol degradation and reducing the release of ROS. This work on topological structure engineering offers new insights into designing high-performance biocatalysts for biological applications.

Due to the accumulation of NADH and ROS during alcohol metabolism, an effective solution is proposed for alcohol detoxification by coenzyme regeneration catalyzed by nanozymes with NADH oxidase-like activity by integrating single-atom Pt into Co3O4 lattices.