Comparative analyses of serum parameters between nontransgenic and transgenic male chickens expressing the 3D8 single-chain variable fragment gene, and mortality and growth characteristics

TL;DR

This study compares the health and growth of transgenic male chickens expressing a specific gene with nontransgenic chickens, finding no adverse effects.

Contribution

The study demonstrates that 3D8 scFv gene expression in male chickens does not negatively impact health or growth metrics.

Findings

Male transgenic chickens had lower levels of several serum markers compared to nontransgenic chickens.

No significant differences in mortality or body weight were observed between transgenic and nontransgenic chickens.

Findings align with previous results in female chickens, suggesting consistent safety of the transgene.

Abstract

Transgenic (TG) animals offer significant potential for diverse applications but may pose risks if the impact of transgene expression on health and physiological parameters is not thoroughly assessed. This study aims to evaluate the effects of 3D8 scFv gene expression on male TG chickens, focusing on key biological markers, mortality, and growth.

Serum samples were collected at 14 weeks of age from male TG and non-TG chickens for comprehensive analysis of serum biochemistry, sex hormones, and cytokine profiles. Mortality and growth were monitored over a 34-week period to assess long-term effects. Statistical comparisons were conducted between TG and non-TG groups to identify significant differences.

The results revealed that male TG chickens showed significantly lower serum levels of alanine aminotransferase, insulin-like growth factor-1, interferon-gamma, interleukin-4, and tumor necrosis factor-1 alpha compared to non-TG chickens (p<0.05). However, no significant differences in mortality or final body weight were observed between groups (p>0.05). These findings are consistent with previous results in female TG chickens, indicating that 3D8 gene expression does not adversely affect health or growth performance.

3D8 scFv gene expression in male TG chickens does not adversely affect mortality, growth, or serum parameters, indicating that the transgene does not have detrimental effects on these critical metrics in male chickens. These findings support the safety and physiological stability of 3D8 gene expression in TG animals.

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