# Isolation and Molecular Detection of Pigeonpox Virus in a Pigeon With Both Cutaneous and Diphtheritic Forms of Pigeon Pox Disease in Ghana

## TL;DR

This study reports the first detection of pigeonpox virus in Ghana, confirming its presence through molecular and histopathological methods.

## Contribution

This is the first documented case of pigeonpox virus in Ghana, highlighting the need for avian disease surveillance.

## Key findings

- PPV was confirmed in a pigeon showing both cutaneous and diphtheritic forms of the disease.
- Molecular analysis showed 100% genetic similarity to the reference PPV isolate FeP2.
- The presence of PPV in Ghana underscores the importance of monitoring avian populations.

## Abstract

Unlike fowl pox, pigeon pox virus (PPV) has not been described in Ghana. This is the first report of the disease and virus in Ghana based on gross necropsy, histopathological analysis, and molecular techniques. This index case was presented as the occurrence of both cutaneous (dry) and diphtheritic (wet) forms of the pigeon pox disease in a 5-month-old female pigeon. Although the observation of Bollinger bodies during histology is confirmatory of pox viruses, the amplification of the p4b gene using polymerase chain reaction and its visualization by gel electrophoresis confirmed the presence of an avian pox virus. Sequencing and phylogenetic analysis of the isolates from this case showed 100% relatedness to the reference PPV isolate FeP2, thus confirming the presence of PPV. The discovery of this virus in Ghana raises the need for surveillance of both wild and domestic avian populations in preparation for any future outbreak, protection of the production birds, and decision on the need for other pox viruses in the country.