Phenotypic and functional comparisons between cryopreserved and freshly isolated peripheral blood mononuclear cells with or without red blood cell lysate (ACK) treatment with special focus on regulatory T cells
Keyvan Habibi, Nils Ågren, Kaoru Okada, Heléne Johansson, Ming Yao, Makiko Kumagai-Braesch
TL;DR
This study compares the effects of cryopreservation on immune cells, focusing on regulatory T cells, and finds that their function is preserved after freezing.
Contribution
The study provides new insights into the impact of cryopreservation and ACK treatment on Treg function and viability.
Findings
ACK-treated PBMCs showed increased IFN-γ-producing cells when stimulated with viral peptides.
Cryopreservation reduced cell viability, CD4+ T-cell population, and FoxP3 expression but preserved Treg function.
Tregs from both fresh and frozen PBMCs equally suppressed PBMC proliferation.
Abstract
Adaptive transfer of autologous regulatory T cells (Treg), or ex vivo-generated immunomodulatory cells, has shown promise in reducing/withdrawing immunosuppression after organ transplantation. The effect of cryopreserving such cells is still unclear. This study aims to evaluate the effects of cryopreservation on the immunomodulatory functions of peripheral blood mononuclear cells (PBMCs) with or without pretreatment with red blood cell (RBC) lysate (ACK). Human PBMCs enriched from buffy coats of healthy blood donors were treated either with ACK or phosphate-buffered saline (PBS). Thereafter, a batch of the PBS-control subset was cryopreserved with 10% dimethyl sulfoxide (DMSO) and subsequently examined for phenotype, functionality, and relative gene expression. We found that ACK-treated PBMCs exhibited higher numbers of interferon gamma (IFN-γ)-producing cells when stimulated with viral…
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Taxonomy
TopicsXenotransplantation and immune response · Immune Cell Function and Interaction · Immunotherapy and Immune Responses