Antigenicity in mice of a recombinant Neisseria gonorrhoeae MafA 2/3 protein
Michael M Girgis, Maria Victoria Humbert, Christopher J McCormick, Myron Christodoulides
TL;DR
This study explores the potential of a Neisseria gonorrhoeae protein as a vaccine candidate by testing its ability to induce immune responses in mice.
Contribution
The study demonstrates that the MafA 2/3 outer membrane protein induces bactericidal antibodies in mice and rabbits, suggesting its potential as a gonococcal vaccine component.
Findings
Antisera from mice immunized with rMafA 2/3 showed bactericidal activity against specific gonococcal strains.
Rabbit antisera to rMafA 2/3 was bactericidal for multiple strains and inhibited bacterial association with human cells.
Allele 88 and 90 MafA 2/3 proteins are common in gonococcal isolates and differ by a single amino acid.
Abstract
There are no prophylactic vaccines for preventing the disease gonorrhea, caused by sexually transmitted infection with the Gram-negative pathogen Neisseria gonorrhoeae. In this study, we examined the antigenicity in mice of a recombinant rMafA 2/3 outer membrane protein (OMP). Mice were immunized with rMafA 2/3 with different adjuvants and delivery vehicles, which induced high levels of antibody that recognized the MafA 2/3 protein in i) antigen and OM-ELISA, ii) OM-western blots and iii) on whole bacteria examined with flow cytometry. Antisera to rMafA 2/3 in liposomes with monophosphoryl lipid A (MPLA) and with Zwittergent 3–14 ± MPLA, were bactericidal in vitro for homologous P9-17 (Allele 193) gonococcal strain (median 50% bactericidal titers of 256). Analysis of MafA 2/3 alleles among gonococcal isolates in the PubMLST database showed that ~50% and 26% of gonococci expressed Allele…
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Taxonomy
TopicsBacterial Infections and Vaccines · Reproductive tract infections research · Microbial infections and disease research