# Building a directed evolution–genome editing pipeline for metabolic traits in specialty crop breeding

**Authors:** Anuran K Gayen, Laura M Carmona Rojas, Zhen Fan, Seonghee Lee, Vance M Whitaker, Andrew D Hanson

PMC · DOI: 10.1093/hr/uhaf203 · 2025-10-25

## Full-text entities

- **Genes:** ARO9 (aromatic-amino-acid:2-oxoglutarate transaminase) [NCBI Gene 856539], ARO8 (bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase) [NCBI Gene 852672]
- **Diseases:** DE (MESH:D051556), GE (MESH:D042822)
- **Chemicals:** anthocyanin (MESH:D000872), GABA (MESH:D005680), lignin (MESH:D008031), tyrosine (MESH:D014443), phenylalanine (MESH:D010649), nitrogen (MESH:D009584), shikimate (MESH:C000723335), NH3 (MESH:D000641), sugar (MESH:D000073893), arogenate (MESH:C015478), DE (-)
- **Species:** Meleagris gallopavo (common turkey, species) [taxon 9103], Cucumis sativus (cucumber, species) [taxon 3659], Oryza sativa (Asian cultivated rice, species) [taxon 4530], Citrus (genus) [taxon 2706], Solanum tuberosum (potatoes, species) [taxon 4113], Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932], Musa acuminata (banana, species) [taxon 4641], watermelon [taxon 260674], Fragaria x ananassa (strawberry, species) [taxon 3747], Escherichia coli (E. coli, species) [taxon 562], Malus domestica (apple, species) [taxon 3750], Glycine max (soybean, species) [taxon 3847], Solanum lycopersicum (tomato, species) [taxon 4081]
- **Mutations:** A G, A118G, A118, phenylalanine/tyrosine, S109I, S109, F137H, F137
- **Cell lines:** FxaPAL1 — Mus musculus (Mouse), Hybridoma (CVCL_C7RB)

## Figures

4 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12574542/full.md

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Source: https://tomesphere.com/paper/PMC12574542