Smo gene silencing: a promising strategy for natural killer/t-cell lymphoma treatment via modulating proliferation and apoptosis
Shengquan Liu, Shaoxiong Wang, Yahong Xu, Yuanling Huang, Pengliang Xin, Yan Zheng, Yishen Wu, Yanling Yang, Xiongpeng Zhu, Chuntuan Li
TL;DR
This study shows that silencing the Smo gene can reduce the growth and increase the death of cancer cells in a type of lymphoma called NKTCL.
Contribution
This is the first study to demonstrate the anti-tumor effects of Smo gene silencing in NKTCL cells.
Findings
Smo gene silencing significantly inhibited the proliferation of NKTCL cells.
Smo silencing increased apoptosis rates in NKTCL cells.
Smo silencing reduced the expression of Gli1 and PD-L1 in NKTCL cells.
Abstract
Natural killer/T-cell lymphoma (NKTCL) is a malignancy with a poor prognosis. The Smoothened (Smo) protein is implicated in NKTCL growth. This study employed lentiviral vector-mediated Smo RNA interference (LV-Smo-RNAi) to silence the Smo gene in the human NKTCL cell line SNT8. Fluorescence microscopy, qRT-PCR, and Western blot verified the reduction of Smo mRNA and protein levels. The CCK-8 assay showed that Smo silencing inhibited cell proliferation. Flow cytometry with annexin V-PE/7-AAD double staining indicated an increased apoptosis rate. Moreover, the expression of GLI family zinc finger 1 (Gli1) and programmed death-ligand 1 (PD-L1) was downregulated. In vivo, xenotransplantation experiments demonstrated that Smo silencing led to slower tumor growth with reduced tumor volume and weight. Overall, Smo gene silencing holds great potential as a novel molecular-targeted therapy…
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Taxonomy
TopicsImmune Cell Function and Interaction · Lymphoma Diagnosis and Treatment · Cell death mechanisms and regulation