Construction of a Prognostic Model of Prostate Cancer Based on Immune and Metabolic Genes and Experimental Validation of the Gene AK5
Wenjie Zhou, Jiawei Ding, Danfeng Xu
TL;DR
This study builds a prostate cancer prognosis model using immune and metabolic genes and experimentally confirms the role of AK5 in tumor growth.
Contribution
A novel prognostic model with ten genes and experimental validation of AK5's oncogenic role in prostate cancer.
Findings
A ten-gene prognostic model was developed and validated across multiple datasets.
AK5 knockdown reduced tumor growth in xenograft and patient-derived xenograft models.
AK5 is significantly expressed in prostate cancer and promotes tumor proliferation.
Abstract
Despite the fact that prostate cancer is one of the most common tumors in men, this study intends to evaluate the predictive significance of immune and metabolic genes in prostate cancer using multi-omics data and experimental validation. The research developed and validated a prognostic model utilizing The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, integrating immune and metabolic gene sets. Additionally, the prognostic gene Adenylate Kinase 5 (AK5) was analyzed in prostate cancer tissue microarrays from Ruijin Hospital. The functional role of the AK5 gene was validated through knockdown and overexpression experiments in four prostate cancer cell lines, employing cell proliferation assays, colony formation assays, and both xenograft models in nude mice and patient-derived xenograft models. This research developed a prognostic model comprising ten genes,…
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Taxonomy
TopicsFerroptosis and cancer prognosis · Cancer, Hypoxia, and Metabolism · Cancer Research and Treatments