# Analysis of Chemical Components and Blood‐Absorbed Components in Youjing Granules by UHPLC‐Q‐Orbitrap‐MS

## TL;DR

This study identifies the chemical components and blood-absorbed substances in Youjing Granules using advanced mass spectrometry techniques.

## Contribution

The study provides a detailed chemical profile and identifies absorbed components in serum after administration of Youjing Granules.

## Key findings

- 132 chemical components were identified, including flavonoids, prenol lipids, and steroids.
- 24 blood-absorbed components were detected, comprising 15 prototypes and 9 metabolites.
- The findings offer insights into the pharmacodynamic basis and mechanism of Youjing Granules.

## Abstract

This study investigates the chemical components of Youjing Granules (YG) and identifies blood‐absorbed components in rat serum following YG administration via gavage. Chemical components and blood‐absorbed components of YG were analysed and identified using ultra‐high‐performance liquid chromatography coupled with hybrid quadrupole‐orbitrap high‐resolution mass spectrometry (UHPLC‐Q‐orbitrap‐MS). Identification was achieved by comparing retention time, precise molecular weight, secondary MS fragments with literature data and reference substances. A total of 132 chemical components were identified and analysed from YG, primarily including flavonoids, prenol lipids, organooxygen compounds, isoflavonoids, steroids and steroid derivatives, as well as cinnamic acids and derivatives. Twenty‐four blood‐absorbed components were detected in serum, comprising 15 prototype components and 9 metabolites. The analysis of chemical components and blood‐absorbed components in YG using UPLC‐Q‐orbitrap‐MS technology provides a reference basis for further elucidating the pharmacodynamic material basis and mechanism of action of YG.