# Investigation of Discoloration of Anterior Teeth With Three Types of Substances Used in Endodontic Treatment

## TL;DR

This study compares how different endodontic materials affect the discoloration of front teeth over time.

## Contribution

The study introduces and evaluates novel sealer formulations containing nano-zinc and nano-copper particles for dental use.

## Key findings

- All tested materials caused statistically significant color changes in teeth over 28 days.
- Sealers containing nano-zinc and nano-copper showed less discoloration than AH26 and MTA Base.
- The novel nano-zinc/copper material had the least color change among tested substances.

## Abstract

This study aims to investigate the discoloration of anterior teeth with three types of substances used in endodontic treatment.

In this laboratory study, 75 newly extracted human anterior (central and lateral) teeth with full roots were examined. This study utilized the following materials: AH26, MTA Base (Maruchi), a novel formulation containing nano‐zinc particles, and a second novel formulation containing both nano‐zinc and nano‐copper particles. The teeth in the first three groups were dried with paper points, and the canals were completely sealed using the sealer lateral filling technique with gutta‐percha. Then the color of the samples was measured by a spectrophotometer at four times: initially, immediately after filling (0), 14 days, and 28 days later.

The results showed that the mean color change (∆E) at all times (immediately, 14 days later, and 28 days later) was statistically significant between all groups (p < 0.05). The degree of color change decreased in the following order: AH26 > Zn‐nano > Zn.Cu‐nano > novel nano‐zinc/copper material > MTA Base (Maruchi) > control group.

The results of the present study showed that sealers with a zinc base (Zn‐nano and Zn.Cu‐nanoparticles) had an appropriate color change. Therefore, they can be used alongside other sealers available in the market.