# Proteome Analysis of the Six-Eyed Sand-Spider Sicarius thomisoides Venom

## TL;DR

This study identifies the main protein components in the venom of the six-eyed sand-spider Sicarius thomisoides, revealing key toxins and allergens.

## Contribution

The first identification of CRISP-type allergens and U-PHTX-Pmx peptides in Sicarius spider venom.

## Key findings

- Phospholipase D family proteins are the majority toxins in S. thomisoides venom.
- Astacin-like metalloproteinases and serine proteases are also present in the venom.
- CRISP-type allergens and U-PHTX-Pmx peptides were identified for the first time in Sicarius venom.

## Abstract

Spiders of the Sicarius genera (Araneae: Sicariidae) are commonly known as six-eyed sand spiders. Of the species described in Latin America, the species S. thomisoides has previously been shown to possess venom with a toxic potential comparable to that observed in the venom of the spider L. laeta. Although identifying the phospholipase D activity in the venom of S. thomisoides, it is still unknown what other components are part of the venom. In this study, we described the identification of the main protein components of S. thomisoides venom, revealing that the phospholipase D family were the majority toxins, followed by Astacin-like metalloproteinases and serine proteases. Additionally, the presence of CRISP-type allergens and peptides from the U-PHTX-Pmx family was identified for the first time in venoms from Sicarius genera. Identifying the components of the Sicarius spider venom is an essential step to understanding its toxicological potential.