# One Bloom Is Not Like the Other—Distinct Environmental Drivers Result in Domoic Acid Events in Monterey Bay, California

## TL;DR

This study explores how different environmental factors lead to domoic acid events in Monterey Bay, using new methods to detect and understand these harmful algal blooms.

## Contribution

The novel use of SPATT for environmental metabolomics reveals new insights into domoic acid events and identifies a potential new marine natural product.

## Key findings

- Two domoic acid events had similar magnitudes but distinct environmental drivers.
- SPATT identified 159 metabolites strongly correlated with domoic acid.
- A new marine natural product was detected via GNPS molecular networking.

## Abstract

Domoic acid (DA), produced by Pseudo-nitzschia diatoms, is the one of the major toxin threats from harmful algal blooms (HABs) on the west coast of the United States. DA events vary in magnitude, timing, and duration, and elucidating drivers for individual events is a persistent challenge. Monterey Bay experiences near-annual DA events and hosts long-term HAB monitoring at the Santa Cruz Municipal Wharf (SCW). Here we characterize two toxin events, occurring in May 2023 and March 2024. The events were similar in magnitude and duration, but an exploration of physical, biological, and chemical dynamics revealed distinct environmental drivers. These differences resulted in a significant deviation in cellular DA (cDA) within the same species of Pseudo-nitzschia. In addition, opportunistic solid-phase adsorption toxin tracking (SPATT) was used for environmental metabolomics. The novel application of SPATT revealed 159 metabolites that were strongly correlated with DA in both events and produced a spectral match to a new marine natural product using Global Natural Products Social Molecular Networking (GNPS). This work takes a multivariable approach to understanding toxin drivers and lends proof of concept for the integration of environmental metabolomics in HAB monitoring.