# JC Virus Agnogene Regulates Histone-Modifying Enzymes via PML-NBs: Transcriptomics in VLP-Expressing Cells

## TL;DR

This study shows that a JC virus gene called agnogene affects enzymes that modify histones in cells, possibly influencing epigenetic regulation and cell cycle control.

## Contribution

The study identifies the agnogene's role in regulating histone-modifying enzymes via PML-NBs, offering new insights into JC virus epigenetic mechanisms.

## Key findings

- The agnogene alters expression of histone-modifying enzymes like EHMT1, PRMT7, KDM2B, KDM5C, and KDM6B.
- Changes in gene expression were more pronounced when the agnogene was present.
- Infected glial cells maintain diploid chromosomes, possibly due to G2 arrest influenced by agnogene activity.

## Abstract

JC virus (JCV) replicates within the nuclei of glial cells in the human brain and causes progressive multifocal leukoencephalopathy. JCV possesses a small, circular, double-stranded DNA genome, divided into early and late protein-coding regions. The non-coding control region (NCCR) functions bidirectionally for both early and late genes, and the agnogene is located downstream of TCR and upstream of three capsid proteins in the late region. Previously, in cell culture systems, we demonstrated that these capsid proteins accumulate in intranuclear domains known as promyelocytic leukemia nuclear bodies (PML-NBs), where they assemble into virus-like particles (VLPs). To investigate the agnogene’s function, VLPs were formed in its presence or absence, and differential gene expression was analyzed using microarray technology. The results revealed altered expression of histone-modifying enzymes, including methyltransferases (EHMT1, PRMT7) and demethylases (KDM2B, KDM5C, KDM6B), as well as various kinases and phosphatases. Notably, CTDP1, which dephosphorylates the C-terminal domain of an RNA polymerase II subunit, was also differentially expressed. The changes were predominant in the presence of the agnogene. These findings indicate that the agnogene and/or its protein product likely influence epigenetic regulation associated with PML-NBs, which may influence cell cycle control. Consistently, in human brain tissue, JCV-infected glial cells displayed maintenance of a diploid chromosomal complement, likely through G2 arrest. The precise mechanism of this, however, remains to be elucidated.