# Genome-Wide Identification and Analysis of the Fatty Acid Export Family Revealed the Role of GmFAX8 in Improving Soybean Oil Accumulation

## TL;DR

This study identifies and analyzes fatty acid export genes in soybeans, finding that GmFAX8 boosts oil accumulation.

## Contribution

The study identifies eight GmFAX genes in soybean and demonstrates that GmFAX8 enhances fatty acid accumulation.

## Key findings

- Eight GmFAX genes were identified in soybean, grouped into four subfamilies.
- GmFAX8 expression is high in leaves and stems, and overexpression increases fatty acid content in hairy roots.
- GmFAX genes contain a conserved Tmemb_14 domain, suggesting functional importance.

## Abstract

Fatty acid transporters (FAXs) play an important role in fatty acid synthesis by facilitating transport fatty acids from the plastid to the endoplasmic reticulum. This process is essential for providing precursor substances necessary for triglycerides (TAGs). Although FAX genes have been identified in variety of plant species, the identification and molecular functions of the GmFAX gene members in soybean are still unclear. In this study, soybean FAX genes were identified through the utilization of the Phytozome (v13) and NCBI online websites. Subsequently, phylogenetic trees, expression patterns, gene structures, and qRT-PCR were analyzed. A total of eight GmFAX members were identified at the whole genome level, and further phylogenetic analysis revealed that these members can be categorized into four subfamilies. In addition, all members of GmFAX contain a highly conserved domain Tmemb_14. Through qRT-PCR analysis, it was found that the expression level of the GmFAX8 gene is relatively high in leaves and stems. Further investigation revealed that the total fatty acid content in hairy roots overexpressing the GmFAX8 gene was significantly greater than that observed in the control strain. The results presented above suggest that the GmFAX8 gene may play an important role in the accumulation of oil within soybeans.