# Molecular and Biochemical Characterization of Xanthomonas arboricola pv. corylina Isolates Infecting Hazelnut Orchards in Chile

## TL;DR

This study characterizes Xanthomonas arboricola pv. corylina isolates from Chilean hazelnut orchards, revealing unique metabolic and genetic traits compared to global isolates.

## Contribution

The Chilean isolates show novel metabolic capabilities and distinct phylogenetic grouping, offering new insights into the pathogen's diversity.

## Key findings

- Chilean isolates can metabolize sorbitol and mannitol, a trait not previously reported in other geographic isolates.
- Multilocus sequence analysis grouped the isolates into four or five clades, differing from international studies.
- Average nucleotide identity analysis confirmed the genetic distinctiveness of the Chilean isolates.

## Abstract

In recent years, the cultivated area of hazelnuts in Chile has increased significantly. Along with this rapid expansion, biotic constraints that affect the optimal development of the crop have been identified. Among these, bacterial blight disease caused by Xanthomonas arboricola pv. corylina has been particularly relevant. This pathogen has a global distribution and is present in all hazelnut-producing countries. In the spring of 2023, hazelnut orchards were sampled from the Maule to Biobío Regions of Chile. The Chilean isolates recovered from hazelnut tissues showing symptoms of bacterial blight were characterized by their ability to grow on different semi-selective media, their carbohydrate utilization profiles, hypersensitivity response in tobacco plants, and biochemical tests. Additionally, the isolates were identified based on the 16S rRNA gene and multilocus sequence analysis (MLSA) on the rpoD, gyrB, and atpD genes. The results showed that the X. arboricola pv. corylina Chilean isolates differed from previously reported isolates in other geographic areas as they are capable of metabolizing sorbitol and mannitol. Using MLSA and average nucleotide identity (ANI) comparison, these isolates were grouped into four and five phylogenetic clades, respectively, representing a significant difference from what has been reported in similar international studies.