# Anti-Photoaging Effects of a Standardized Hot Water Extract of Petasites japonicus Leaves in Ultraviolet B-Exposed Hairless Mice

## TL;DR

A hot water extract from Petasites japonicus leaves reduces UVB-induced skin aging in hairless mice by improving hydration, reducing wrinkles, and protecting collagen.

## Contribution

This study demonstrates the anti-photoaging effects of a standardized Petasites japonicus leaf extract in a UVB-exposed mouse model.

## Key findings

- KP-1 reduced wrinkle formation, epidermal thickening, and transepidermal water loss in UVB-exposed mice.
- KP-1 inhibited MMP-1 and enhanced COL1A1, while modulating MAPK/AP-1 and TGF-β/Smad2/3 signaling pathways.
- KP-1 balanced p53/p21 expression and restored cyclin D1 and CDK4 levels to prevent cellular senescence.

## Abstract

Background: Ultraviolet B (UVB) radiation accelerates skin aging by inducing oxidative stress, collagen degradation, and cellular senescence. Although Petasites japonicus is known for its antioxidant properties, its anti-photoaging potential remains underexplored. This research explored the protective properties of a hot water extract from P. japonicus leaves (KP-1) against photoaging caused by UVB exposure. Methods: Hairless mice were exposed to UVB three times per week and orally administered KP-1 for 13 weeks. Wrinkle formation, epidermal thickness, skin hydration, and collagen content were assessed. Protein expression related to MAPK/AP-1, TGF-β/Smad2/3, and p53/p21 pathways was analyzed by Western blotting. Results: KP-1 significantly reduced UVB-induced wrinkle area, epidermal and dermal thickening, and transepidermal water loss while restoring collagen density and skin hydration. KP-1 inhibited MMP-1 expression, enhanced COL1A1 levels, suppressed MAPK/AP-1 activation, and activated TGF-β/Smad2/3 signaling. It also balanced p53/p21 expression and restored cyclin D1 and CDK4 levels, thereby preventing UVB-induced senescence. Conclusions: The findings of this research revealed that KP-1 can serve as a promising natural substance for safeguarding the skin from damage and aging caused by UVB exposure.