# Genome-Wide Identification of Novel miRNAs and Infection-Related Proteins in Leishmania major via Comparative Analysis of the Protozoa, Vectors, and Mammalian Hosts

## TL;DR

This study identifies new microRNAs and infection-related proteins in Leishmania major, offering potential targets for treating leishmaniasis.

## Contribution

The study presents novel miRNAs and infection-related proteins in Leishmania major through comparative genomic analysis.

## Key findings

- 2963 conserved proteins were identified across L. major, its vector, and mammalian hosts.
- 27 infection-related proteins, including 24 protein kinases, were discovered as potential therapeutic targets.
- 29 novel miRNAs in L. major were identified, with seven absent in all mammalian species.

## Abstract

Leishmania major is a unicellular protozoan that causes cutaneous leishmaniasis in mammals and is mainly transmitted by the sand fly Phlebotomus papatasi. However, the contribution of microRNAs (miRNAs) and protein-coding genes to its pathogenic mechanisms remains largely unexplored. In this study, we systematically analyzed miRNAs and protein-coding genes in L. major, its insect vector, and mammalian hosts. Comparative genomic analysis revealed 2963 conserved proteins shared among the three groups, highlighting a core set of proteins across protozoa, vectors, and hosts. Among mammals, human proteins exhibited the highest homology with L. major, while P. papatasi displayed the lowest proportion of homologs. Functional annotation of 94 hypothetical proteins identified 27 infection-related proteins, including 24 protein kinases and three tyrosine phosphatases, which may represent novel therapeutic targets. In addition, an EST-based approach identified 29 novel miRNAs in L. major. Phylogenetic analysis indicated that these miRNAs diverged into two distinct evolutionary branches, and homology analysis revealed that seven miRNAs were absent in all mammalian species. For example, miR-10117-3p was detected only in nematode Heligosmoides polygyrus. Furthermore, miRNA-gene interaction network analysis highlighted four key genes potentially involved in L. major infection. Collectively, our findings expand current knowledge of protozoan virulence by identifying novel miRNAs and infection-related proteins and provide promising candidates for future drug development against leishmaniasis.