# Effect of Acidic Environment and Tooth Brushing on the Color and Translucency of 3D-Printed Ceramic-Reinforced Composite Resins for Indirect Restorations and Hybrid Prostheses

## TL;DR

This study tested how acidic environments and tooth brushing affect the color and translucency of 3D-printed dental materials used in restorations.

## Contribution

The study introduces a novel comparison of 3D-printed ceramic-reinforced composite resins and milled resins under simulated oral conditions.

## Key findings

- Acidic environments and tooth brushing did not significantly affect color stability of the tested materials.
- Translucency decreased significantly for all materials after immersion and brushing.
- 3D-printed materials showed lower translucency compared to milled resins.

## Abstract

This study evaluated the effect of acidic environment and tooth brushing on the color stability and translucency of stained 3D-printed ceramic-reinforced composite (CRC) resins for indirect restorations and hybrid prostheses. Twelve specimens were prepared from each 3D-printing resin material: Ceramic Crown (CC), OnX (ONX), and Tough 2 (T2), and one CAD/CAM milling resin, Lava Ultimate (LU). After preparation, all specimens were stained, then immersed in either water or citric acid. Subsequently, the specimens underwent simulated tooth brushing for 3650 cycles. Color stability (ΔE) and translucency parameter (TP) were measured using a spectrophotometer. Data were analyzed using ANOVA, post hoc Tukey tests, and independent Student t-tests (α = 0.05). Material type, immersion medium, and their interaction did not significantly influence the mean ΔE (p > 0.05). The lowest ΔE value was for LU in acid (ΔE = 1.11 ± 0.39), and the highest for T2 in water (ΔE = 2.09 ± 1.47). Except for ONX and LU in acid, all materials had ΔE values above the perceptibility threshold (ΔE = 1.2). The mean TP was significantly affected by material type, immersion medium, and their interaction (p < 0.05). The lowest TP value was for group CC in acid (0.91 ± 0.26); the highest was for group LU in acid (6.24 ± 0.56). After immersion and subsequent tooth brushing, TP values decreased for all materials. Exposure to an acidic environment and tooth brushing did not affect color stability but significantly reduced translucency. Both the 3D-printed CRCs and milled resin material displayed comparable color stability below clinically acceptable thresholds, though the translucency of 3D-printed materials remained lower compared to milled material.