# Synergistic Effects of Nitrogen Application Enhance Drought Resistance in Machilus yunnanensis Seedlings

## TL;DR

Adding nitrogen, especially a mix of ammonium and nitrate, helps Machilus yunnanensis seedlings resist drought by improving soil and plant health.

## Contribution

The study demonstrates that mixed nitrogen application is more effective than single forms in enhancing drought resistance in ornamental tree seedlings.

## Key findings

- Drought stress acidifies soil and reduces key nutrients like organic matter and phosphorus, impairing plant growth.
- Ammonium nitrogen outperforms nitrate in mitigating drought effects, with mixed nitrogen showing the best overall performance.
- Mixed nitrogen improves soil pH, nutrient availability, and plant water use efficiency, while boosting antioxidant activity.

## Abstract

Drought poses a severe challenge to ornamental tree growth under climate change. This study employed a 2 × 4 factorial design—with two soil moisture levels (80–85% vs. 50–55% field capacity) and four nitrogen treatments (NN: no nitrogen; NO: nitrate nitrogen; NH: ammonium nitrogen; MN: mixed nitrate-ammonium nitrogen)—to examine the efficacy of nitrogen addition in enhancing drought resistance in Machilus yunnanensis seedlings. Results revealed that (1) drought stress leads to the acidification of rhizosphere soil, resulting in a decrease of 7.67%, 29.51%, 14.07%, and 44.09% in the content of soil organic matter (SOM), available phosphorus (AP), available potassium (AK), and dissolved organic nitrogen (DON), respectively. This adverse change directly impacts plant growth; it is manifested by a significant reduction of 45% in total chlorophyll (T Chl), a 67.18% decrease in photosynthetic rate (Pn), as well as reductions of 10.61%, 27.59%, 14.81%, and 12.35% in plant height, leaf, stem, and total biomass, respectively. (2) The application of all three forms of nitrogen helps alleviate drought stress, as evidenced by the recovery of photosynthetic levels and the reduction in malondialdehyde (MDA) content, with ammonium-N exhibiting superior efficacy over nitrate-N across most metrics. (3) Strikingly, the mixed nitrogen form outperformed singular applications by demonstrating multifaceted advantages: It maintains soil pH levels and rhizosphere nutrient availability under drought conditions, particularly with a 10.99% and 33.44% increase in dissolved organic nitrogen and available phosphorus content, respectively. More importantly, under drought stress, it increased leaf water content by 20.31%, nitrogen use efficiency by 15.67%, and photosynthetic nitrogen use efficiency by 439.44%, promoted the accumulation of osmolytes, while upregulating antioxidant enzyme activity to counteract osmotic imbalance and alleviate oxidative damage. These findings highlight that nitrogen supplementation, particularly mixed nitrogen application, enhances drought resistance in M. yunnanensis, offering a viable management strategy to sustain urban tree landscapes in water-limited environments.