# Antiviral Activity of Glucosyl Hesperidin Against Feline Calicivirus

## TL;DR

This study shows that glucosyl hesperidin can inhibit feline calicivirus without harming cells, suggesting it may help control norovirus.

## Contribution

The study demonstrates glucosyl hesperidin's antiviral activity against FCV as a potential natural norovirus suppressant.

## Key findings

- GH inhibited FCV replication in a concentration-dependent manner with an IC50 of 3281 μM.
- GH showed high biocompatibility with 100% cell viability at concentrations up to 8000 μM.
- Complete viral inhibition was not achieved at the highest concentration tested.

## Abstract

The objective of this study was to evaluate the antiviral activity of glucosyl hesperidin (GH), a water-soluble derivative of hesperidin with known antioxidant and anti-inflammatory properties, in order to explore its potential applications. Antiviral activity was assessed using feline calicivirus (FCV), a surrogate model for human norovirus, a major foodborne pathogen. Cytotoxicity testing in Crandell–Rees feline kidney (CRFK) cells demonstrated that GH exhibited high biocompatibility, maintaining 100% cell viability at concentrations up to 8000 μM. Antiviral efficacy assays revealed that GH inhibited FCV replication in a concentration-dependent manner across the range of 250~8000 μM, with a half-maximal inhibitory concentration (IC50) of 3281 μM. Complete viral inhibition, however, was not achieved at the maximum concentration tested. In conclusion, GH was shown to inhibit FCV while maintaining low cytotoxicity, indicating its potential as a natural, water-soluble candidate for the suppression of norovirus.