# Synthesis of Poly(Lactic Acid-co-Arginine) and Construction of Its Ternary Phase Diagram for Nonsolvent Induced Phase Separation

## TL;DR

Researchers synthesized a new biocompatible polymer by combining lactic acid and arginine, and created a phase diagram to guide its use in making nanoparticles and membranes.

## Contribution

The novel contribution is the synthesis of poly(lactic acid-co-arginine) and the construction of its ternary phase diagram for nonsolvent-induced phase separation.

## Key findings

- PLAA contains –NH2, –NH–, and NH= groups in its molecular chain.
- PLAA is an amorphous copolymer.
- The ternary phase diagram identifies three distinct regions for processing PLAA materials.

## Abstract

L-arginine, a basic amino acid, exhibits high biocompatibility, reactivity, and absorbability. It was selected as the co-polymer modification monomer for L-lactic acid with the objective of enhancing the hydrophilicity of poly(lactic acid) (PLA), neutralizing the acidity of PLA degradation products, and regulating the degradation cycle. The copolymer poly(lactic acid-co-arginine) (PLAA) was synthesized by direct melting polycondensation of L-arginine and L-lactic acid, and the structures and properties of PLAA were characterized. The results indicated the presence of –NH2, –NH–, and NH= in the molecular chain of the copolymer PLAA. Furthermore, the PLAA was identified as an amorphous copolymer. The “PLAA/CHCl3/C6H14” ternary phase diagram was constituted by nonsolvent-induced phase separation (NIPS) by selecting chloroform (CHCl3) as a good solvent and n-hexane (C6H14) as a nonsolvent. The phase diagram displays three distinguishable regions: the homogeneous zone, the metastable zone, and the phase separation zone. These regions are identified by the binodal and spinodal curves. The ternary phase diagram establishes a theoretical foundation for the preparation and processing of PLAA nanoparticles, composite materials, and porous fibers or membranes.