# Herbicidal Control Potential of the Endophytic Bacterium B. pseudorignonensis BFYBC-8 Isolated from E. crus-galli Seeds

## TL;DR

A new eco-friendly bioherbicide based on the bacterium B. pseudorignonensis BFYBC-8 shows strong weed suppression without harming crops.

## Contribution

Identification of B. pseudorignonensis BFYBC-8 as a novel bioherbicide candidate with high efficacy and crop safety.

## Key findings

- B. pseudorignonensis BFYBC-8 reduced E. crus-galli plant height by 16.7% and root length by 85.1%.
- The bacterial strain colonized E. crus-galli roots and showed thermostable inhibitory activity.
- The bioherbicide had no toxicity to rice, wheat, and corn crops.

## Abstract

The long-term application of traditional chemical herbicides has caused a significant escalation in herbicide resistance of barnyard grass (Echinochloa crus-galli). As an eco-friendly alternative, biological herbicides demonstrate substantial application potential. Acknowledging the growing herbicide resistance of E. crus-galli, this study aimed to screen target bacteria with inhibitory effects on the growth for bio-herbicide development. By using ungerminated E. crus-galli seeds as the screening substrate, a bacterial strain (BFYBC-8) with potent inhibitory activity was isolated and identified as Brucella pseudorignonensis. Pot experiments revealed that inoculation with B. pseudorignonensis BFYBC-8 significantly suppressed E. crus-galli growth, reducing plant height by 16.7% and root length by 85.1%, while markedly inhibiting biomass accumulation. Fluorescent labeling with green fluorescent protein (GFP) showed that BFYBC-8 successfully colonized the root intercellular spaces of E. crus-galli and extended continuously along the tissue matrix. Additionally, the strain’s supernatant metabolic products exhibited exceptional thermostability: inhibitory activity against E. crus-galli was maintained after thermal treatment at 28 °C, 60 °C, 80 °C, and 100 °C. Crucially, the bacterium displayed no toxicity to agronomically important crops such as rice, wheat, and corn. This study highlights B. pseudorignonensis BFYBC-8 as a promising candidate for bioherbicide development and provides an important reference for applying seed-associated pathogenic bacteria in developing bioherbicides for sustainable weed management.