# Assessing the Impact of a Novel Trichoderma sp. Strain STP8 on Lettuce Yield and Mineral Content

## TL;DR

A new strain of Trichoderma fungus boosts lettuce yield and mineral content in field conditions, showing promise as a sustainable biofertilizer and biocontrol agent.

## Contribution

The study introduces Trichoderma sp. STP8 as a novel native strain with significant yield improvement in field-grown lettuce.

## Key findings

- Application of Trichoderma sp. STP8 increased lettuce yield by 16.6% to 30.5% in field conditions.
- The highest yield gains occurred when the strain was applied before head formation, 26 days after planting.

## Abstract

The fungal genus Trichoderma is highly valued in agriculture for its versatile roles, mainly as a biocontrol agent against plant pathogens. Recently, its use as a natural biofertilizer has gained attention, as Trichoderma spp. promotes crop growth and improves yield by enhancing the rhizosphere environment and activating plant defences. Globally, over 250 Trichoderma-based products dominate 60–90% of the market, but their efficacy can decline during transportation and storage. Additionally, concerns about their impact on native soil biodiversity have led to interest in using locally adapted, native strains. The novel native strain of Trichoderma sp. STP8 (formerly T. koningiopsis agg. STP8) previously showed strong antagonism against Sclerotinia sclerotiorum and promoted lettuce growth in greenhouse conditions. This study evaluated Trichoderma sp. STP8’s effectiveness in field-grown lettuce, revealing yield increases of 16.6% to 30.5%. The most significant gains occurred when Trichoderma sp. STP8 was applied before head formation, 26 days after planting. That was in one treatment with two applications (at seedling planting and after 26 days) and another with three applications (at sowing, at seedling planting, and after 26 days). These results demonstrate Trichoderma sp. STP8’s potential as a sustainable biocontrol and biofertilizer agent for lettuce, encouraging further research across different agricultural systems, including hydroponics and soil-less medium.