# A Chronocosmetic Approach to Treating Signs of Aging with Glutathione

## TL;DR

This study explores using glutathione with a timed skincare routine to reduce aging signs like wrinkles and uneven skin tone.

## Contribution

A novel chronocosmetic protocol combining topical and oral glutathione with microneedling is introduced for anti-aging.

## Key findings

- Improvements in pigmentation, radiance, and expression lines were observed after 8 weeks.
- The treatment was well-tolerated, including for Fitzpatrick skin types III–IV.

## Abstract

Cutaneous aging is associated with oxidative stress and visible changes on the skin, such as uneven pigmentation, wrinkles, and loss of radiance. Glutathione (GSH), an endogenous antioxidant with anti-melanogenic properties, is considered a promising active agent in anti-aging protocols. This study evaluated a chronocosmetic approach based on the combined application of topical glutathione with microneedling, oral supplementation (250 mg), and a day-and-night home care routine. An 8-week interventional study was conducted with seven participants aged 30 to 60 years. Glutathione was applied in clinic using nanoneedles, along with a daily home care routine and oral administration. The progression of aging signs was assessed using 3D imaging, clinical photography, and cosmetic dermatology evaluation. The results showed improvements in pigmentation, radiance, and expression lines, with good tolerability, even in Fitzpatrick skin types III–IV.