# Advances in Genetic Engineering Techniques for Improved Forest Trees: Applications in Biomass, Stress Resilience and Carbon Sequestration

**Authors:** Sophia Hydarry Matola, Jingjing Li, Meiou Sun, Lu Yang, Wenhui Zhuang, Jingli Yang

PMC · DOI: 10.3390/ijms262010192 · International Journal of Molecular Sciences · 2025-10-20

## TL;DR

This paper reviews how genetic engineering is being used to improve forest trees for traits like faster growth, stress resistance, and better carbon storage.

## Contribution

The paper provides a comprehensive review of recent advances in genetic engineering techniques for forest trees, emphasizing novel applications and challenges.

## Key findings

- CRISPR-based editing and multi-omics approaches are enabling precise trait modifications in trees.
- GMTs show promise for enhancing wood quality, drought tolerance, and carbon sequestration.
- Ecological and regulatory challenges remain significant barriers to the deployment of genetically modified trees.

## Abstract

Forest biotechnology is rapidly advancing from conventional breeding toward molecular design, enabling the development of genetically modified trees (GMTs) with traits such as accelerated growth, stress resilience, and improved wood properties. This review systematically examines recent breakthroughs in tree genetic engineering, beginning with traditional methods and progressing to CRISPR-based precision editing and multi-omics-guided trait design. We highlight applications in wood quality (e.g., lignin reduction in Populus spp.), drought tolerance (e.g., PagHyPRP1 and PtoMYB142 editing), phytoremediation (e.g., heavy metal accumulation in poplar), and carbon sequestration. We also evaluate ecological and socio-regulatory challenges, including gene flow risks and public acceptance. Based on this integrated analysis, we outline future directions for responsible deployment of GMTs in sustainable forestry and global carbon neutrality efforts.

## Full-text entities

- **Chemicals:** Carbon (MESH:D002244), lignin (MESH:D008031), heavy metal (MESH:D019216)
- **Species:** Populus (poplar, genus) [taxon 3689]

## Full text

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## Figures

7 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12564089/full.md

## References

154 references — full list in the complete paper: https://tomesphere.com/paper/PMC12564089/full.md

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Source: https://tomesphere.com/paper/PMC12564089