# Review of Microchip Analytical Methods Coupled with Aptamer-Based Signal Amplification Strategies for High-Sensitivity Bioanalytical Applications

## TL;DR

This review discusses how combining aptamers with microchip technology improves the sensitivity and efficiency of detecting bioanalytical substances.

## Contribution

The paper provides a comprehensive review of aptamer-based signal amplification strategies integrated with microchips for bioanalytical applications.

## Key findings

- Aptamer-based biosensors offer high affinity and selectivity for target detection.
- Microchip platforms enhance detection through ease of operation and high throughput.
- Signal amplification strategies improve sensitivity in biomedical and environmental applications.

## Abstract

Aptamers have many advantages, including facile synthesis and a high affinity and good selectivity toward their targets. Therefore, aptamer-based biosensors have become increasingly popular for the detection of different bioanalytical substances. Microchip-based analytical detection platforms offer significant advantages for the detection of different analytes, including their ease of operation, high throughput, cost-effectiveness, and high sensitivity. Aptamer-based signal amplification techniques have been combined with microchips to sensitively detect bioanalytical substances due to their stable reactions, easy operation, and specificity in biomedical science and environmental fields. This review summarizes representative articles about aptamer signal amplification strategies on microchips for the detection of bioanalytical substances, as well as their advantages and challenges for specific applications. We highlight the accomplishments and shortcomings of aptamer signal amplification strategies on microchips and discuss the direction of development and prospects of aptamer signal amplification strategies on microchips.