Cinnamomum migao H.W. Li Ethanol-Water Extract Suppresses IL-6 Production in Cardiac Fibroblasts: Mechanisms Elucidated via UPLC-Q-TOF-MS, Network Pharmacology, and Experimental Assays
Yuxin Lu, Yaofeng Li, Can Zhu, Mengyue Guo, Xia Liu, Xiangyun Chen
TL;DR
This study identifies how an extract from Cinnamomum migao suppresses IL-6 in heart cells, potentially offering a new treatment for heart fibrosis.
Contribution
The study reveals the active compounds and molecular mechanisms of Cinnamomum migao extract in inhibiting cardiac fibroblast IL-6 production.
Findings
MG-EWE contains 173 compounds, with 14 core constituents targeting IL-6 synthesis via ADRB2 and MAPK9.
Laurolitsine and Hecogenin inhibit CF proliferation, migration, and IL-6 expression through the ADRB2/JNK/c-Jun pathway.
Experimental assays confirm MG-EWE's anti-fibrotic effects by suppressing ISO-induced CF transdifferentiation.
Abstract
This study aims to elucidate the active components and underlying molecular mechanisms by which the ethanol-water extract of Cinnamomum migao H.W. Li (MG-EWE) inhibits cardiac fibroblast (CF) transdifferentiation and IL-6 production, providing insights into its anti-myocardial fibrosis effects. The chemical composition of MG-EWE was characterized using UPLC-Q-TOF-MS. Network pharmacology analysis identified active constituents and their mechanisms in inhibiting IL-6 production in CFs. An isoproterenol (ISO)-induced rat CF model was established to evaluate the effects of MG-EWE and its main monomers, Laurolitsine and Hecogenin, on cell proliferation, migration, collagen metabolism, IL-6 production, and key proteins in the ADRB2/JNK signaling pathway. A total of 173 compounds were identified in MG-EWE, with 14 core constituents regulating IL-6 synthesis via 16 key targets, including ADRB2…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsCardiac Fibrosis and Remodeling · Signaling Pathways in Disease · Phytochemicals and Medicinal Plants