# Genome-Wide Association Study of Morphological Defects in Nellore Cattle Using a Binary Trait Framework

## TL;DR

This study identifies genetic markers linked to morphological defects in Nellore cattle, offering insights for breeding improvements.

## Contribution

The study applies a binary trait GWAS to identify novel candidate genes for morphological defects in Nellore cattle.

## Key findings

- Significant associations were found for four morphological traits: feet and legs, chamfer, hump, and loin.
- Candidate genes were enriched in functions like bone remodeling, muscle development, and lipid metabolism.
- Overlaps with QTL for conformation, feed intake, and carcass quality were observed.

## Abstract

Background/Objectives: Morphological defects such as limb malformations, cranial asymmetries, loin deviations, jaw misalignments, and navel irregularities are associated with early culling and reduced productivity in beef cattle. In Bos taurus indicus such as Nellore, the genetic basis of these traits remains poorly characterized. This study aimed to investigate the genetic architecture of six morphological defects in Nellore cattle, namely feet and legs malformation, chamfer asymmetry, fallen hump, loin deviation, jaw misalignment, and navel irregularities, via a genome-wide association study (GWAS) approach tailored for binary traits. Methods: Depending on the trait, the number of genotyped animals analyzed ranged from 3369 to 23,206, using 385,079 SNPs (after quality control). Analyses were conducted using a linear mixed model framework adapted for binary outcomes. Results: Significant associations were identified for four traits: feet and legs, chamfer, hump, and loin. No significant markers were detected for jaw or navel defects, likely due to lower sample sizes and trait incidence. Gene annotation revealed 49 candidate genes related to feet and legs, 4 for chamfer, 4 for hump, and 6 for loin. Conclusions: Candidate genes were enriched for biological functions, including bone remodeling, muscle development, lipid metabolism, and epithelial organization. Overlaps with QTL related to conformation, feed intake, reproductive traits, and carcass quality were also observed. These findings provide novel insights into the genetic control of morphological defects in Nellore cattle and may inform breeding strategies aimed at improving structural soundness.