# Preventive and Therapeutic Effects of Hydrogen-Generating Si-Based Agent on Pressure Ulcers in Mice

## TL;DR

A new silicon-based agent that generates hydrogen was found to reduce pressure ulcers in mice by lowering inflammation and oxidative stress.

## Contribution

A novel Si-based agent that continuously generates hydrogen for treating pressure ulcers was tested in a mouse model.

## Key findings

- The Si group showed significantly smaller ulcer areas and faster healing compared to the control group.
- Inflammatory responses and oxidative stress were significantly reduced in the Si group.
- Apoptotic activity in ulcer tissue was suppressed in mice given the Si-based agent.

## Abstract

Objectives: As a known antioxidant, hydrogen has been useful for treating pressure ulcers. However, conventional methods of hydrogen administration have limitations with regard to dosage and continuity of hydrogen intake. This study evaluated the efficacy of a novel Si-containing agent that can generate substantial quantities of hydrogen to treat pressure ulcers in an in vivo mouse model. Methods: The back skin and subcutaneous tissue of mice were compressed with magnets for 12 h. Changes in the ulcer area after release of compression, histological findings, degree of apoptosis, and expression levels for oxidative stress markers and inflammation-related cytokines were compared between mice fed a normal diet (control group) and those fed a 2.5 wt% Si-based diet (Si group). Results: The Si group had a significantly smaller ulcer area and shorter healing period than the control group. Moreover, inflammatory responses, apoptotic activity, and oxidative stress within the ulcer tissue were suppressed significantly in the Si group. Conclusions: Oral intake of the Si-based agent can potentially treat and prevent pressure ulcers by regulating apoptosis, oxidative stress, and inflammatory responses.