# Influence of Cutting Styles on Antioxidant Capabilities of Fresh-Cut Cauliflower by Regulating ROS Metabolism and Antioxidant Enzyme Activity

**Authors:** Qihan Guo, Bingheng Li, Jiarui Wang, Minke Shi, Jiayu Wang, Yan Chen, Yunjie Zhang, Sarengaowa, Ying Xiao, Ke Feng

PMC · DOI: 10.3390/antiox14101188 · Antioxidants · 2025-09-28

## TL;DR

This study shows how different cutting methods affect the antioxidant capacity of fresh-cut cauliflower during storage.

## Contribution

The study reveals how cutting styles influence antioxidant enzyme activity and ROS metabolism in fresh-cut cauliflower.

## Key findings

- Cutting style 4 (CS4) showed the highest increase in antioxidant capacity compared to other styles.
- Phenolic compound accumulation increased significantly in all cutting styles after 72 hours of storage.
- Antioxidant enzyme activities, such as catalase and superoxide dismutase, were enhanced by specific cutting methods.

## Abstract

The enzymatic browning and oxidative deterioration of cauliflower during mechanical processing are major challenges for the fresh-cut cauliflower industry. The primary objective of this study was to investigate the impact of cutting styles on the antioxidant capacity of fresh-cut cauliflower during storage. One flower (12 × 1.8 cm) of cauliflower was designated as cutting style 1 (CS1). CS1 was cut longitudinally into strips as cutting style 2 (CS2). CS1 was also cut transversely into cubes, as cutting style 3 (CS3), and longitudinally and transversely into small cubes, as cutting style 4 (CS4). Results indicated that at the conclusion of the 72 h storage period, cutting treatments enhanced the total antioxidant capacity of fresh-cut cauliflower in the ABTS assay by 128.1%, 82.9%, 50.1%, and 38.9% for CS1, CS2, CS3, and CS4, respectively. All treatment groups except CS1 exhibited increased total antioxidant capacity in the FRAP assay. Phenolic compound accumulation increased by 106.82%, 105.24%, 270.4%, and 295.3% in CS1, CS2, CS3, and CS4, respectively. In addition, the O2−· scavenging activity was enhanced; the activities of antioxidant-related enzymes, including catalase (CAT) and superoxide dismutase (SOD), were also increased. In conclusion, the extent of the effect on antioxidant capacity was as follows: CS4 > CS3 > CS2 > CS1. This study has elucidated the patterns of influence exerted by cutting methods upon the quality of fresh-cut cauliflower, thereby providing theoretical foundations and empirical data to inform the selection of appropriate cutting techniques for both commercial processing and domestic culinary applications.

## Linked entities

- **Proteins:** Cat (Catalase)

## Full-text entities

- **Genes:** SOD1 (superoxide dismutase 1) [NCBI Gene 6647] {aka ALS, ALS1, HEL-S-44, IPOA, SOD, STAHP}, CAT (catalase) [NCBI Gene 847]
- **Chemicals:** O2- (-), ABTS (MESH:C002502)
- **Species:** Brassica oleracea var. botrytis (cauliflower, varietas) [taxon 3715]

## Full text

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## Figures

11 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12561630/full.md

## References

60 references — full list in the complete paper: https://tomesphere.com/paper/PMC12561630/full.md

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Source: https://tomesphere.com/paper/PMC12561630