# Curcumin-Functionalized Ag and ZnO Nanoparticles: A Nanotherapeutic Approach for Treating Infections in Diabetic Wounds

## TL;DR

This paper introduces a new wound dressing using curcumin-functionalized Ag-ZnO nanoparticles that effectively treats infections in diabetic wounds and accelerates healing.

## Contribution

The novel use of curcumin-functionalized Ag-ZnO nanoparticles for treating diabetic wound infections is introduced and validated through preclinical and clinical studies.

## Key findings

- Curcumin-functionalized Ag-ZnO nanoparticles inhibited multidrug-resistant pathogens like MRSA within 60 seconds.
- The treatment significantly reduced healing time in patients compared to standard care and unloaded nanoparticle controls.
- Molecular docking simulations confirmed curcumin's high affinity for Staphylococcus aureus Protein A.

## Abstract

Chronic wounds, such as diabetic ulcers, remain a significant clinical challenge due to high infection risk and delayed healing. This study presents a comprehensive evaluation of a novel wound dressing incorporating curcumin-functionalized silver–zinc oxide (Ag-ZnO) nanoparticles. The formulation was rationally designed based on molecular docking simulations that identified curcumin as a high-affinity ligand for Staphylococcus aureus Protein A. The synthesized nanoparticles demonstrated potent, broad-spectrum antibacterial activity, achieving complete inhibition of multidrug-resistant pathogens, including MRSA, within 60 s. A critical comparative assessment, incorporating an unloaded Ag-ZnO nanoparticle control group, was conducted in both a rabbit wound model and a randomized clinical trial (n = 75 patients). This design confirmed that the enhanced wound-healing efficacy is specifically attributable to the synergistic effect of curcumin combined with the nanoparticles. The curcumin-loaded Ag-ZnO treatment group showed a statistically significant reduction in healing time compared to both standard care and unloaded nanoparticle controls (e.g., medium wounds: 19.6 days vs. 90.6, p < 0.001). These findings demonstrate that curcumin-functionalized Ag-ZnO nanoparticles offer a safe and highly effective therapeutic strategy, providing robust antibacterial action and significantly accelerated wound healing.