# Off-label Use of Gastrointestinal Multiplex PCR Panel for the Diagnosis of Amoebic Liver Abscess: A Three-case Pediatric Report

## TL;DR

A test designed for gut infections was used off-label to quickly diagnose liver abscesses in three children, showing promise for resource-limited areas.

## Contribution

Demonstrates the off-label use of a gastrointestinal PCR panel for diagnosing amoebic liver abscess in pediatric patients.

## Key findings

- The PCR panel provided rapid and accurate diagnosis of amoebic liver abscess in three pediatric cases.
- This approach enabled timely antiamoebic treatment, which is critical for patient outcomes.
- The unvalidated use of the panel for this purpose shows potential but requires further study.

## Abstract

Diagnosis of amoebic liver abscess is challenging. A multiplex polymerase chain reaction panel, traditionally employed for the detection of enteric pathogens in stool, proved useful for the rapid diagnosis in 3 pediatric patients when used off-label on liver abscess fluid. While this approach enabled timely and targeted antiamoebic therapy, highlighting its potential in high-burden, resource-limited settings, it represents an unvalidated use of the technology that requires further investigation.