# Cordypyridones E–J: Antibiofilm 2‑Pyridone Alkaloids from the Nematode Antagonistic Fungus Laburnicola nematophila

## TL;DR

Scientists discovered new 2-pyridone alkaloids from a fungus that fights nematodes, with some showing strong antibiofilm and antimicrobial properties.

## Contribution

The discovery of six new cordypyridone alkaloids and the identification of potent antibiofilm activity in two of them.

## Key findings

- Compound 4 showed broad-spectrum bioactivity in cytotoxicity and antimicrobial assays.
- Compounds 5 and 6 significantly reduced Staphylococcus aureus biofilm formation by nearly 50% at low concentrations.

## Abstract

In the course of biochemical prospection
of Laburnicola
nematophila isolated from eggs of the plant-parasitic
cyst nematode Heterodera filipjevi,
eight 2-pyridone alkaloids were isolated from its solid-state BRFT
cultures and identified as six previously undescribed, cordypyridones
E–J (1–6), and two known congeners,
cordypyridones C (7) and D (8). The structures
and absolute configurations of all isolated compounds were elucidated
through HR-ESI–MS, 1D/2D NMR spectroscopy, and TDDFT–ECD
calculations. Compound 4, bearing a rare N-hydroxy-2-pyridone moiety structurally related to that of PF1140,
exhibited broad-spectrum bioactivity in cytotoxicity and antimicrobial
assays. Compounds 5 and 6 demonstrated potent
antibiofilm activities against Staphylococcus aureus, reducing its biofilm formation by almost 50% at 0.25 and
7.8 μg/mL, respectively.