# Structural causes of pattern formation and loss through model-independent bifurcation analysis

## TL;DR

This paper introduces a new framework to study how cell patterns form during development using minimal assumptions about cell communication and signaling.

## Contribution

The novel contribution is a model-independent ODE framework that predicts cell patterns based on general assumptions about communication and signaling.

## Key findings

- Global intercellular communication networks determine possible emergent patterns in a generic system.
- Adding constraints on intracellular signaling leads to a single stable pattern based on network features.
- The framework allows inferring unknown signaling interactions from tissue-level patterns.

## Abstract

During development, precise cellular patterning is essential for the formation of functional tissues and organs. These patterns arise from conserved signaling networks that regulate communication both within and between cells. Here, we develop and present a model-independent ordinary differential equation (ODE) framework for analyzing pattern formation in a homogeneous cell array. In contrast to traditional approaches that focus on specific equations, our method relies solely on general assumptions about global intercellular communication (between cells) and qualitative properties of local intracellular biochemical signaling (within cells). Prior work has shown that global intercellular communication networks alone determine the possible emergent patterns in a generic system. We build on these results by demonstrating that additional constraints on the local intracellular signaling network lead to a single stable pattern which depends on the qualitative features of the network. Our framework enables the prediction of cell fate patterns with minimal modeling assumptions, and provides a powerful tool for inferring unknown interactions within signaling networks by analyzing tissue-level patterns.