High genomic diversity of Vibrio parahaemolyticus from underexplored tropical northern Australia: a baseline for future surveillance

TL;DR

This study explores the genomic diversity of Vibrio parahaemolyticus in tropical northern Australia, revealing high variability and potential pathogenic traits that highlight the need for comprehensive surveillance.

Contribution

The study provides the first genomic analysis of Vibrio parahaemolyticus from underexplored tropical northern Australia, revealing novel pathogenic and genomic features.

Findings

Northern Australian Vibrio parahaemolyticus strains show genomic diversity and intermix with Asian and South American isolates.

A gastrointestinal isolate harbored a unique TRH2 toxin variant and a plasmid with a Vibrio alginolyticus pilT copy.

Plasmid diversity in clinical isolates suggests mobile elements play a role in adaptability and virulence.

Abstract

High genomic diversity of Vibrio parahaemolyticus from underexplored tropical northern Australia: genomic plasticity with pathogenic potential demands a One Health surveillance approach.

Vibrio parahaemolyticus is an autochthonous marine bacterium that causes gastroenteritis after ingestion of raw or undercooked seafood and, less frequently, wound and tissue infections. Genomic information from northern Australia is scarce, and most sequences to date stem from foodborne outbreaks in southern states. We analysed 24 environmental and 5 clinical isolates (4 wound and 1 gastrointestinal case) collected in the wet–dry tropics from northern Australia and placed them alongside 48 representative public genomes. Core-genome phylogeny showed that the northern Australian strains sit within the broad VppAsia lineage and intermix with Asian and South American isolates, reflecting the limited geographic structuring in the marine environment. A gastrointestinal isolate (RDH3, ST-2901) harboured the trh2-positive VPaI-β pathogenicity island (including vtrAB and hlyDBAC genes) and displayed an A187S change in the TRH2 toxin which was absent in the other TRH-1/-2 sequences of the isolates analysed in this study. Wound isolates lacked tdh and trh genes, suggesting that the species’ intrinsic virulome may suffice for tissue colonization; notably, one wound strain carried a plasmid with a Vibrio alginolyticus pilT copy linked to twitching motility which has been associated with wound infections. It remains to be determined whether this additional pilT copy, beyond the core genome copies, confers a selective advantage for tissue colonization. Plasmid diversity amongst clinical isolates and a diseased aquaculture fish, including a putative chimaera plasmid in the gastrointestinal isolate, underscores the role of mobile elements as reservoirs allowing adaptability to changing environments. Our findings expand the Australian genomic catalogue beyond outbreak strains, reveal extensive accessory-genome variability in tropical waters and underscore the need for One-Health surveillance frameworks that monitor virulence and resistance markers beyond the canonical hemolysin genes.

Figures