Dynamic Equilibria between DNA-Stabilized Silver Nanoclusters and Silver-Carrying DNA Strands
Cecilia Cerretani, Donato Ranieri, Giacomo Romolini, Christian Brinch Mollerup, Letizia Liccardo, Elena-Alexandra Niţă, Loredana Latterini, Tom Vosch
TL;DR
This paper studies how DNA-stabilized silver nanoclusters interact with DNA strands, showing how their optical properties change with temperature and concentration.
Contribution
The study reveals dynamic equilibria between DNA-AgNCs and Ag+-carrying DNA strands, showing how their interactions affect photophysical properties.
Findings
Three distinct species of 13mer-AgNCs were identified based on concentration and temperature.
The 16mer-stabilized AgNCs show temperature-dependent emission changes but no concentration-dependent shifts.
Aggregation of (16mer)2-[Ag20]10+ was observed through time-resolved anisotropy.
Abstract
DNA-stabilized silver nanoclusters (DNA-AgNCs) are versatile emitters whose photophysical properties are defined by the DNA template. In this study, we explore the unusual temperature- and concentration-dependent behavior of two NIR-emissive DNA-AgNCs: one stabilized by two 16-base DNA oligomers (16mer), and another embedded within a 3-base shortened version of these strands (13mer). Using a combination of optical spectroscopy and mass spectrometry, we show that Ag+-carrying DNA strands can reversibly attach to DNA-AgNCs and fine-tune their photophysical properties. For 13mer-AgNC, we demonstrate the presence of three different species, (13mer)2-[Ag20]10+, (13mer)3-[Ag27]17+, and (13mer)4-[Ag34]24+, depending on concentration and temperature. For the AgNC stabilized by two 16-base DNA strands, (16mer)2-[Ag20]10+, emission wavelength and intensity vary with temperature, although no…
Genes, proteins, chemicals, diseases, species, mutations and cell lines named across the full text — each resolved to its canonical identifier and authoritative record.
Click any figure to enlarge with its caption.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7Peer Reviews
No public reviews on file for this paper yet. If you reviewed it on a platform where reviews are public (OpenReview, ICLR, NeurIPS, ICML), you can paste yours below so the community can read it here.
Videos
No videos yet. Explain this paper in a talk, walkthrough, or lecture? Add one.
Taxonomy
TopicsNanocluster Synthesis and Applications · Gold and Silver Nanoparticles Synthesis and Applications · Advanced biosensing and bioanalysis techniques