The directionality of collective cell delamination is governed by tissue architecture and cell adhesion in a Drosophila carcinoma model
Marta Mira-Osuna, Steffen Plunder, Eric Theveneau, Roland Le Borgne
TL;DR
This study shows how tissue structure and cell adhesion control the direction in which cancer cells detach from tissues in a fruit fly model.
Contribution
The study reveals how septate junctions and cell adhesion influence delamination direction in a Drosophila carcinoma model.
Findings
Loss of septate junctions in RasV12-cells promotes collective delamination.
Apical and basal delamination differ in cell identity and junctional remodeling.
Tissue architecture and preferential adhesion regulate delamination direction.
Abstract
Epithelial cells contact each other and the extracellular matrix via cell junctions, establishing mechanochemical barriers. During collective delamination, epithelia-derived tumors detach from the tissue with a directionality that dictates their malignancy. How cell junctions contribute to this process and how the directionality of delamination is regulated remains unknown. We used the Drosophila RasV12 carcinoma model and found that the loss of septate junctions in epithelial cells triggers apoptosis, whereas in RasV12-cells promotes collective delamination. We found that apical and basal delamination differ in cell identity, polarity, and junctional remodeling, occurring exclusively in squamous and pseudostratified epithelia, respectively. We performed mathematical simulations using a 2D agent-based model and found that tissue architecture and preferential adhesion between mutant…
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Taxonomy
TopicsCellular Mechanics and Interactions · Mathematical Biology Tumor Growth · Viral Infectious Diseases and Gene Expression in Insects