# Genome-wide identification and expression analysis of the CesA/Csls gene family in Eucalyptus Grandis

## TL;DR

This study identifies and analyzes the CesA/Csls gene family in Eucalyptus grandis, revealing their roles in cell wall formation and cellulose synthesis.

## Contribution

The first comprehensive identification and functional analysis of the CesA/Csls gene family in Eucalyptus grandis.

## Key findings

- 62 CesA/Csls genes were identified and classified into seven subfamilies in Eucalyptus grandis.
- Genes EgCesA4, EgCesA7, and EgCesA8 are highly expressed in xylem, suggesting roles in cellulose synthesis during lignification.
- Promoter regions of CesA/Csls genes contain abundant light-responsive cis-acting elements.

## Abstract

The cellulose synthase gene superfamily is composed of two major gene families: cellulose synthase (CesA) and cellulose synthase-like (Csl). These genes play a crucial role in the synthesis of cellulose and hemicellulose within the plant cell wall, and are essential for controlling plant growth and development. However, the CesA/Csls gene family has not been previously reported in Eucalyptus grandis.

In this study, bioinformatics methods were employed to identify the CesA/Csls gene family inE. grandisand to analyze the potential functions of its members in cell wall formation.

The results revealed that there were 62 CesA/Csls family members inE. grandis, which were classified into seven subfamilies (CesA, CslA, CslB, CslC, CslD, CslE, CslG) based on evolutionary tree analysis. Promoter regions of these genes contained various cis-acting elements, with light-responsive elements being the most abundant. Gene expression pattern analysis showed that EgCesA4, EgCesA7, and EgCesA8 were highly expressed in the xylem, suggesting their primary association with cellulose synthesis during secondary wall thickening (lignification).

Overall, the analysis of the EgCesA/Csls gene family provides a valuable reference for understanding cellulose synthesis in the cell wall, genetic improvement, and biotechnological applications.