Transcriptomic and physiological analyses revealed nicotianamine enhances wheat tolerance to excess manganese
Daozhen Luo, Qing Li, Fei Pang, Wenjie Zhang, Muhammad Usman, Yangrui Li, Yongxiu Xing, Dengfeng Dong
TL;DR
The study shows that nicotianamine helps wheat tolerate excess manganese by boosting its production and regulating metal balance.
Contribution
The novel contribution is identifying specific nicotianamine-related genes that enhance wheat's tolerance to excess manganese.
Findings
Mn-tolerant wheat genotypes show increased nicotianamine (NA) production and gene expression.
Exogenous NA application reduces Mn uptake and restores essential cation homeostasis in wheat.
Candidate genes TaNAS, TaNAAT, TaYSL2, and TaYSL6 are linked to Mn tolerance in wheat.
Abstract
Manganese (Mn) is an essential plant micronutrient but toxic at supra-optimal concentrations. Although nicotianamine (NA) biosynthesis is metal inducible, its specific role in Mn detoxification in wheat remains poorly characterized. Integrated transcriptomic and physiological analyses of Mn-tolerant (ET8, Carazinho) and Mn-sensitive (ES8, Egret) wheat near-isogenic lines and their parental cultivars under excess Mn revealed pronounced upregulation of genes encoding nicotianamine synthase (TaNAS), nicotianamine aminotransferase (TaNAAT), and putative Mn-NA transporters (TaYSL2, TaYSL6) in tolerant genotypes. This transcriptional response correlated with elevated NA accumulation in roots under Mn stress. Exogenous NA application enhanced Mn tolerance in hydroponically grown seedlings in a concentration-dependent manner, accompanied by reduced tissue Mn accumulation, and maintained…
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Taxonomy
TopicsCrop Yield and Soil Fertility · Heavy Metals in Plants · Plant Micronutrient Interactions and Effects