Kinetic mechanisms of electron bifurcation with electron transfer flavoprotein, NADH, butyryl-CoA dehydrogenase, and ferredoxin reveal a semiquinone cycle

TL;DR

This study reveals how electron bifurcation works in a bacterial enzyme system, using a cycle involving flavins and temperature-dependent reactions.

Contribution

The paper introduces a semiquinone cycle mechanism for electron bifurcation and shows how temperature affects the reaction rate.

Findings

Bcd releases the inhibition of α-FAD•−, enabling fast β-FAD reduction.

Two simultaneous one-electron transfers from EtfAHQB reduce Bcd and form EtfASQB.

The reaction rate increases 14-fold at 30 °C, the optimal growth temperature of A. fermentans.

Abstract

Electron transfer flavoprotein (EtfAB, with α-FAD and β-FAD) and tetrameric butyryl-CoA dehydrogenase (Bcd, with δ-FAD in each subunit) from Acidaminococcus fermentans catalyze electron bifurcation which reduces low potential ferredoxin (Fd) and high potential crotonyl-CoA using NADH as an electron donor. Our previous rapid kinetic studies have demonstrated “pseudo-electron bifurcation” where NADH and two EtfAB molecules generate EtfASQB (ASQ contains α-FAD•−) and the charge-transfer complex of EtfASQBHQ:NAD+ (BHQ contains β-FADH−). Since the radical in EtfASQB inhibits the further reduction of β-FAD with NADH, the question arises as to how the five components of the complete system interact to mediate the whole flavin-based electron bifurcation. This study shows that Bcd releases the inhibition effect of α-FAD•−, allowing fast β-FAD reduction for turnover. In the presence of both Bcd and Fd, the total β-FADH− of EtfAB bifurcates to afford α-FAD•− and Fd−; a second bifurcation yields α-FADH− in the Bcd-EtfAHQB complex and additional Fd−. In the presence of crotonyl-CoA, two simultaneous one-electron transfers from both EtfAHQB yield reduced Bcd and two EtfASQB, confirmed by electron paramagnetic resonance spectroscopy. This step is proposed to require a slow conformational change of the Bcd-EtfAB complex for electron transfer with a limiting rate constant of 0.0098 s−1 at 4 °C, but increases about 14-fold to 0.14 s−1 at 30 °C, the optimal growth temperature of A. fermentans. The final reduction of crotonyl-CoA to butyryl-CoA completes the cycle, which we call the semiquinone cycle of electron bifurcation, because it starts and ends with a semiquinone.

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