# Nutritional Profile and Sensory Acceptance of Sourdough Breads Enriched With Ora‐pro‐nóbis Leaf Powder

**Authors:** Dayane Lilian Gallani Silva, Filipe Andrich, Barbara Daniele Almeida Porciuncula, Marcelo Augusto Batista, Marcela Moreira Terhaag, Beatriz Cervejeira Bolanho Barros

PMC · DOI: 10.1111/1750-3841.70629 · 2025-10-24

## TL;DR

This study shows that adding ora-pro-nóbis leaf powder to sourdough bread improves its nutrition and is well accepted by consumers.

## Contribution

The study introduces a sustainable method to enhance sourdough bread's nutritional value using ora-pro-nóbis leaf powder.

## Key findings

- SB5 had 10% more protein and 90% more dietary fiber than the control.
- Enriched breads showed higher calcium, magnesium, phenolics, and antioxidant activity with increased OPNP.
- SB3 was most recommended due to its superior nutrition and consumer purchase intention.

## Abstract

This study aimed to characterize the composition of ora‐pro‐nóbis leaf powder (OPNP) and evaluate the nutritional profile of enriched sourdough breads (SBs). Four SB formulations were prepared using different concentrations of OPNP (SBC, 0; SB1, 1%; SB3, 3%; and SB5, 5%) and a leavening agent (a mixture of lactic acid bacteria and yeast). SBs were evaluated for physicochemical, antioxidant, and sensory properties. OPNP was shown to contain 21.01 g 100 g−1 proteins, 30.92 g 100 g−1 dietary fiber, 3.28 g 100 g−1 calcium, and 69.42 mg 100 g−1 total phenolic compounds. Seven phenolic acids and six flavonoids were identified in OPNP. The leavening agent used in SBs production contained 7.40 log colony‐forming units (CFU) g−1 lactic acid bacteria and 4.98 log CFU g−1 yeasts. SB5 had higher contents of proteins (10%) and dietary fibers (90%) than SBC. There was a gradual increase in calcium, magnesium, phenolics, and antioxidant activity as OPNP concentration increased. Enriched SBs were darker and greener than SBC. By contrast, hardness, elasticity, and chewiness did not differ significantly between formulations. In a hedonic test conducted with consumers, enriched SBs received similar scores for aroma, flavor, and texture. This study presents a sustainable strategy for incorporating non‐conventional food plants into bakery products, with SB3 being the most recommended formulation, owing to its superior nutritional profile compared with SBC and SB1 and higher purchase intention than SB5.

## Full-text entities

- **Chemicals:** SB1 (MESH:C047101), flavonoids (MESH:D005419), Ora-pro-nobis (-), SB5 (MESH:D000068879), magnesium (MESH:D008274), phenolic acids (MESH:C017616), calcium (MESH:D002118)
- **Species:** Saccharomyces cerevisiae (baker's yeast, species) [taxon 4932]

## Figures

2 figures with captions in the complete paper: https://tomesphere.com/paper/PMC12552800/full.md

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Source: https://tomesphere.com/paper/PMC12552800