# Neuroprotective effects of candesartan in 3-nitropropionic acid-induced Huntington’s disease: modulation of angiotensin and CREB/BDNF/PGC1-α signaling

## TL;DR

Candesartan, a drug that blocks a specific receptor, shows neuroprotective effects in a Huntington's disease model by improving brain function and reducing inflammation.

## Contribution

This study demonstrates that candesartan mitigates 3NP-induced Huntington’s disease through activation of neuroprotective signaling pathways.

## Key findings

- Candesartan improved memory, motor, and cognitive functions in rats with Huntington’s disease.
- The drug activated the Ang II/AT2R/Ang-(1-7)/Mas receptor axis and increased neurotrophic proteins like CREB/BDNF/PGC1-α.
- Candesartan reduced inflammation and mitochondrial dysfunction while inhibiting harmful signaling pathways like JNK/c-Jun.

## Abstract

Renin–Angiotensin System has been implicated in neurodegenerative diseases such as Huntington’s (HD). It is made up of two axes: one is the Angiotensin II Type 1 receptor (AT1R) or Angiotensin II Type 2 receptor (AT2R), while the other is angiotensin-(1-7) [Ang-(1-7)], and Mas receptor; the latter has reported developing a neuroprotective effect; oppositely, AT1R activation has been linked to neurodegenerative diseases. This study aims to elucidate the potential neuroprotective effect of Candesartan (Cand) an AT1R blocker in mitigating neuronal degeneration caused by 3-Nitropropionic acid (3NP) induced HD by revealing the prospective role of Ang II/AT2R/Ang-(1-7)/Mas receptor, CREB/BDNF/PGC1-α besides JNK/c-Jun trajectories, as well as the anti-apoptotic survivin. HD was induced by 3NP (10 mg/kg) for 14 days. Rats received Candesartan (2.5 or 5 mg/kg) for 14 days, after which brain and striatum were isolated for the histopathological, immunohistochemical, and biochemical analysis. Cand displayed significant improvement in the rats’ behavioral tests, enhancing their memory, motor, and cognitive functions induced by 3NP, which was confirmed by the striatal histopathological and immunohistochemical examination of the GFAP. In addition, Cand activated the Ang II/AT2R/Ang-(1-7)/Mas receptor axis. Moreover, Cand stimulated the production of striatal neurotrophic proteins CREB/BDNF/PGC1-α which in turn decreased the levels of inflammatory mediators NF-κB and IL-1β, accompanied by an increase in the antioxidants NQO1 and HO-1 levels. Similarly, Cand led to the inhibition of the JNK/c-Jun with activation of survivin. In conclusion, Candesartan has mitigated the striatal degeneration and mitochondrial dysfunction induced by 3NP through various mechanistic pathways.

The online version contains supplementary material available at 10.1007/s10787-025-01889-6.