# Efficient Agrobacterium‐Mediated Transformation of Green Arabidopsis Suspension Cells

## TL;DR

Researchers developed a highly efficient method to transform green Arabidopsis suspension cells using Agrobacterium, enabling large-scale genetic studies and compound production.

## Contribution

A novel Agrobacterium-mediated transformation protocol achieving nearly 100% infection rates in green Arabidopsis suspension cells.

## Key findings

- Using AGL1 strain and solidified medium plates increased transformation efficiency to nearly 100%.
- AB minimal salts and Pluronic F68 were critical for successful transformation.
- The method enables high-throughput genetic manipulation for industrial and pharmaceutical applications.

## Abstract

Photosynthetic plant cell suspension cultures are a valuable experimental system for analyzing various physiological processes. This system bypasses the structural complexity of the whole plant organism and can be manipulated under uniform conditions. However, achieving a highly efficient and consistent transformation of plant suspension cells remains challenging. By using green fluorescent protein (GFP) and a microplate confocal imaging system for high‐throughput analysis, we optimized the transformation of green Arabidopsis suspension cells to infect almost 100% of the cells. Key elements of our protocol included using the hypervirulent Agrobacterium tumefaciens strain AGL1, co‐cultivating agrobacteria and suspension cells on solidified medium plates, and adding AB minimal salts and the surfactant Pluronic F68. The presented method can significantly increase the transformation rate of plant suspension cells, facilitating the introduction of genetic pathways for producing industrial, cosmetic, or pharmaceutical compounds in these systems.

We present a highly efficient and reproducible Agrobacterium‐mediated transformation protocol for photosynthetic Arabidopsis suspension cells, achieving infection rates of almost 100% within 5 days.